Showing posts with label Volumetric. Show all posts
Showing posts with label Volumetric. Show all posts

Graduated Cylinder And Volumetric Flask




The image is Pixabay property

One of the tools used to measure liquids in the lab is a graduated cylinder. Graduated cylinder has tube shaped with a holder on the bottom as a glass holder. Graduated cylinder size assortment among which 5 ml, 10 ml, 20 ml, 25 ml, 50 ml, 100 ml, 500 ml, 1000 ml, 2000 ml, and so forth. Graduated cylinder has an accuracy under the burette, volumetric pipette, and graduated pipette. Graduated cylinder has a scale from top to bottom, from a scale of 0 (zero) ml to its highest volume, for example 10 ml graduated cylinder, has a scale of 0 ml to 10 ml. At the top there is a glass teapot spout such as water, to facilitate the discharge of the graduated cylinder.

Graduated cylinder is made of glass or plastic for their intended use. Graduated cylinders made of glass can be used for all kinds of liquids such as acids, bases, salts, and distilled water. But graduated cylinders made of plastic can only be used for neutral liquids such as saline and distilled water. It is better not to use plastic graduated cylinder to take acid or base for plastic could possibly react to acid or base. How to use a graduated cylinder that is clean glassware washing with water and soap, brush the inside of the graduated cylinder with tube brush, then rinse with water. Dry the graduated cylinder or rinse with distilled water 3 times. Rinse the graduated cylinder with a liquid to be measured 3 times, so that the concentration of the liquid is not reduced because it is mixed with distilled water.

The use of graduated cylinders very easy, much easier than burette or pipette, ie pour the liquid into a graduated cylinder, remove excess liquid, then measure the desired fluid.  For example the fluid is needed as much as 5 ml, then pour the liquid into a graduated cylinder 5 ml, then discard the liquid if it exceeds a scale of 0 ml. If the difficulty to get a liquid that fits on a scale of 0 ml, using a pasteur pipette. Pasteur pipette is a tool shaped glass small tube that pointed lower end and the upper end of a rubber such as a small oval balloon. Pasteur pipette doesn’t have scales. Rinse the pasteur pipette with distilled water 3 times and then with the liquid 3 times.

How to get distilled water or a liquid with a pipette that is the input end of a glass pipette into distilled water or liquids, squeeze the bubble gum and then release. When the rubber bubble is released, the liquid will rise into the pipette. Lift the pipette from the liquid and then wipe with a tissue or blotting paper, try with one wipe, the outside of the pipette becomes dry. Then place the pipette in a graduated cylinder, 1 cm above the liquid. And push the bubble gum slowly, this will cause a discharge from a pipette in the form of liquid droplets.

If the fluid is aligned with a scale of 0 ml. Stop droplets and reinsert the residual liquid in the pipette into the bottle of fluid. Then pour the liquid from the graduated cylinder into the next container. Wash the graduated cylinder with water back, then soap and water. Then dry. Do not forget to wash the pipette which has been used by opening the bubble gum, and a small pipe pipette was washed with water, then with soap, and finally with water. And dry it.

In addition to graduated cylinders, glassware others are used to obtain or create a solution in accordance with the desired volume accurately that volumetric flask. Volumetric flask used to prepare a solution or liquid whose concentration can be known for certain. This solution is made from solid or liquid substances whose concentration is concentrated. Volumetric flask had various sizes of 5 ml, 10 ml, 20 ml, 25 ml, 50 ml, 100 ml, 200 ml, 250 ml, 500 ml, 1000 ml and so on. Volumetric flask made of glass, the bottom is round but not perfectly round, and there is a pipe extending into it. At the top of the pipe there is a line that marks the boundary of the volume of the volumetric flask.

Volumetric flask must first be cleaned with water and soap, rub the inside of the tube by using a brush, the last rinsed again with water. Volumetric flask should not be dried by putting in the oven, because it expands and the volume will be increased so that the volume, then measurement becomes inaccurate.

Before use, rinse the volumetric flask 3 times with distilled water. For example, to create na2so4 salt solution, weigh as much as 1.2345 grams of salt na2so4 with weighing bottle using an analytical balance. Take a clean volumetric flask and then place on top of the funnel glass (insert the tip of the funnel into the volumetric flask). Among the funnel and flask, place tissue so that air can get out of the volumetric flask when inserting liquid into it. Open the weighing bottle cap and rinse 3 times with distilled water, fill the rinse water weighing bottle cap into the weighing bottle, do not let the rinse water that falls out.

Enter the distilled water into the bottle weigh up to ¾ of the weighing bottle. Stir this solution with stir bar glass until all the salt dissolved in distilled water. Then enter this salt solution into the volumetric flask through the funnel, by way of stick rod funnel drop of fluid in the weighing bottle with stir bar glass. The liquid in the weighing bottle will flow through the stir bar glass then through the funnel and into the flask. This is done so that no liquid falls out of the flask when poured from weighing bottle. Rinse the weighing bottle with distilled water 3 times. Rinse water and insert into the volumetric flask with stirring bar and funnels.

Rinse the stir bar glass 3 times with distilled water so that the rinse water above the funnel into the volumetric flask. Rinse the funnel three times with distilled water and rinse water enter into the volumetric flask. Do not forget to rinse the funnel outer pipe that had been entered into the volumetric flask. Then rinse the pipe wall volumetrik flasc three times with distilled water. Add distilled water up to 1 cm below the line volume volumetric flask.

Dry the inside flask wall above the line volume using tissue and stir a bar glass. Take distilled water with a pasteur pipette and then push the rubber pasteur pipette in the pipeline volumetric flask, so that the droplets of distilled water in a volumetric flask hatch. Apply a drop of distilled water it until it reaches the line marked with the volume of solution basin bottom volumetric flask volume to reach the line. Take the cap volumetric flask is dried and then close the volumetric flask.

Take a volumetric flask with one hand where the index finger attached to the lid. Paste this index finger firmly to prevent leakage current in shake volumetric flask. Lift the flask with all the fingers except the index finger. Turn the flask until air bubbles there are positioned the bottom of the flask. Then flip it over again until the bubble goes to the top of the flask with an index finger remained attached to the lid. Do this 10 times. Then it's done, dissolving salt na2so4 in distilled water using a volumetric flask. The concentration of this solution can be calculated.

Burette, Volumetric Pipette, and Graduated Pipette



The image is Pixabay property

In the lab the fluid is of various kinds, there are acids, bases, salts, distilled water. Each type of liquid has the properties of each are different. The tools used to measure the fluid is made of glass that is resistant to all the properties of a liquid. There are various kinds of glass measuring tools, starting from the accurate to the technical. Measurement tools among which the burette, volumetric pipette, and graduated pipettes.

Burette is a measure of a liquid's most accurate. Burette has a volume of 25 ml, 50 ml, and more. Burette used for titration process. Titration process is the addition of a reagent to the reagent that will cause a reaction. Burette has a scale like the ruler which can read up to two decimal places, for example 5.24 ml. To determine the last digit readings in Burette liquid that is a curved line that is inside the Burettete, there are two lines are above and below, read the scale on the Burettete with respect to the curved lines below. Burette usually used for liquids that have a low concentration, for example 0.1003 M.

The Burette shape is long like a pipeline of approximately 30 cm for Burette 25 ml and 60 cm for Burette 50 ml. There is a scale of 1 ml to 25 ml or 50 ml from top to bottom. Among a scale of 1 ml to 2 ml and so there are 10 scales which indicates 0.1 ml to 0.9 ml. Among a scale of 0.1 ml and 0.2 ml indicating the centerline of 0.05 ml. So we can read the Burette to 2 decimal places. At the end there is a small tap Burette like tap water. This faucet is used to open and close the Burette. At the time of the Burette open, fluid in the Burette going out or drip. When used in the process of titration, Burette position upheld straight. If Burette in a state tilted the volume of liquid coming out of the tap Burette will not be accurate and the titration process must be repeated. To enforce the Burette, plumb worn clamp embedded in the poles. Tip of the clamp with rubber so Burette will not be broken when clamped by the clamp.

To use the Burette, make sure the Burette is clean and has been washed. Rinse the inside Burette with distilled water 3 times and remove the distilled water through the taps. Before introducing the liquid into the Burette, rinse the inside of the Burette with liquid and remove liquid through the tap. This is done 3 times so that the liquid is put into the Burette is not reduced concentration because it is mixed with distilled water rinses the rest of the first. To insert fluid into the Burette, Burette taps cap and place a funnel at the top Burette fouled with blotting paper or tissue so that no air can come out of the Burette when the fluid entry.

Enter liquids with 100 ml beaker into the Burette through the funnel. Enter to exceed the scale of the Burette. Place a beaker containing the remainder of the fluid before it under the tap Burette. Open the tap Burette so that the liquid flows into a beaker. There must be no air bubbles on the top or bottom of the faucet tap. If all parts of the Burette was filled with liquid, then close the faucet Burette. Reinsert the existing liquid in a beaker of 100 ml under the tap Burette into the Burette through the funnel up over the scale of the Burette.

Lift the funnel and blotting paper, being careful not to blotting paper into the Burette. Note the liquid over the Burette scale and open the faucet slowly Burette so that the liquid drops. Close the faucet Burette if the liquid has reached a scale of 0.00 ml Burette. At the time of titration, at the base of a pillar or under the tap Burette, must be laid white tile or white paper so that the color change signaled the end of the titration process becomes clear.

Pipette have a level of accuracy under the Burette. Pipette used to measure fluid that Volumetric pipette and Graduated pipette. Volumetric pipette is small pipe and has a middle section oval tube as goiter. Volumetric pipette size assortment among which 1 ml, 2 ml, 5 ml, 10 ml, 20 ml, 25 ml, 50 ml and so on. Fluids are measured with the volumetric pipette is very accurate for example, if it took 5 ml of fluid with volumetric pipette 5 ml, the liquid coming out of the volumetric pipette exactly 5 ml. On top of the pipette Volumetric there is dark or blue line indicating the boundary volume pipette. Volumetric pipette tip taper so that the liquid that comes out easily in one direction.

Graduated pipette shaped like a straight pipe. Graduated pipette assortment of sizes among which 5 ml, 10 ml, 15 ml and others. The accuracy of a graduated pipette is lower when compared with the volumetric pipette. In the pipette there is a scale to 0.05 ml, for example, in graduate pipette 10 ml, there is a scale of 1 ml to 10 ml. Among a scale of 1 ml and 2 ml contained a scale of 0.1 ml to 0.9 ml. With a graduated pipette, the liquid can be measured up to 2 decimal places, for example 4.25 ml. This graduated pipette as Burette but do not have a tap at the bottom. Graduated pipette tip taper as volumetric pipette so that the liquid out easily in one direction.

How to use a pipette to take a liquid both for volumetric pipette and graduated pipette is the pipettes must be clean and have been washed and dried. If the pipette is not dry, then rinse the pipette with distilled water 3 times. And rinse with a liquid to be taken 3 times as well. This is done so that the liquid does not mix with distilled water and the concentration is reduced. After the Pipetteerballon plug at the top of the pipettes. Pipetteerballon made of rubber that is ball with three pipes. The first pipe at the top pipetteerballon to remove the air from the ball. In the pipeline there is a triangular arrow. The second pipe at the bottom like the letter t tiny of the pipetteerballon is used to draw air and suck fluids, other pipe serves to reduce the liquid. In each of the pipes there are arrows.

Attach a pipette on the bottom of pipetteerballon, enter into a pipe. And the exhaust air from the pipetteerballon by means of a ball is pressed together with the arrows on the pipe at the top of the pipetteerballon. So that the pipetteerballon will shrivel. Wipe the outside of the pipette from top to bottom using a tissue or blotting paper to dry, try do in one wipe. Open the lid of the bottle of liquid to be taken, then insert the pipette into it. Suction fluid by a squeeze of arrows on the pipe with a rubber ball that is inserted pipette.

The liquid will rise into the pipette and pipetteerballon balls will be filled with air. Take up liquid over the scale of the pipette. Then lift the pipette from the liquid and wipe the outside of the pipette from top to bottom using a tissue. Try doing in one wipe, so that pipette becomes dry. And there is no liquid from the outside pipette down and increase the volume of fluid. Place the pipette on the bottle neck where fluid before and tilt fluid bottle 45 ○ with left hand, if the right hand holding a pipette. Or vice versa.

Enforce pipettes, pipette along with Pipetteerballon hold with one hand, the index finger and thumb holding a sign triangular arrow on the pipetteerballon to reduce the liquid. Press the arrow so that the liquid down into the bottle neck. Eyes fixed on the movement of fluids, do not pass on a scale of 0.00 ml pipette. There are two curved lines inside the pipette, stop the decline of this fluid if the bottom has a curved line on a scale of 0.00 ml.

Take the pipette and still hold together with a pipetteerballon, while the hand holding the bottle neck enforce bottles and caps with a lid. For example the liquid to be taken is 5.55 ml. Take an empty 100 ml beaker with a hand that was holding the bottle and enforce the pipette. Tilt the beaker 45 ○ like the neck of the bottle. Lower fluid to 5.55 ml. The rest of the liquid in the pipette is inserted back into the bottle. Pipette which has been used should be washed again with water and then with soap and finally with water until clean. The most important part is cleaned inside the pipette. Do not dry in the oven for pipette, pipettewill expands, thereby reducing the accuracy of the pipette.

In the lab The fluid is of various kinds, there are acids, bases, salts, distilled water. Each type of liquid has the properties of each are different. The tools used to measure the fluid is made of glass that is resistant to all the properties of a liquid. There are various kinds of glass measuring tools, starting from the accurate to the technical. Measurement tools among which the burette, volumetric pipette, and graduated pipettes.

Burette is a measure of a liquid's most accurate. Burette has a volume of 25 ml, 50 ml, and more. Burette used for titration process. Titration process is the addition of a reagent to the reagent that will cause a reaction. Burette has a scale like the ruler which can read up to two decimal places, for example 5.24 ml. To determine the last digit readings in Burette liquid that is a curved line that is inside the Burettete, there are two lines are above and below, read the scale on the Burettete with respect to the curved lines below. Burette usually used for liquids that have a low concentration, for example 0.1003 M.

The Burette shape is long like a pipeline of approximately 30 cm for Burette 25 ml and 60 cm for Burette 50 ml. There is a scale of 1 ml to 25 ml or 50 ml from top to bottom. Among a scale of 1 ml to 2 ml and so there are 10 scales which indicates 0.1 ml to 0.9 ml. Among a scale of 0.1 ml and 0.2 ml indicating the centerline of 0.05 ml. So we can read the Burette to 2 decimal places. At the end there is a small tap Burette like tap water. This faucet is used to open and close the Burette. At the time of the Burette open, fluid in the Burette going out or drip. When used in the process of titration, Burette position upheld straight. If Burette in a state tilted the volume of liquid coming out of the tap Burette will not be accurate and the titration process must be repeated. To enforce the Burette, plumb worn clamp embedded in the poles. Tip of the clamp with rubber so Burette will not be broken when clamped by the clamp.

To use the Burette, make sure the Burette is clean and has been washed. Rinse the inside Burette with distilled water 3 times and remove the distilled water through the taps. Before introducing the liquid into the Burette, rinse the inside of the Burette with liquid and remove liquid through the tap. This is done 3 times so that the liquid is put into the Burette is not reduced concentration because it is mixed with distilled water rinses the rest of the first. To insert fluid into the Burette, Burette taps cap and place a funnel at the top Burette fouled with blotting paper or tissue so that no air can come out of the Burette when the fluid entry.

Enter liquids with 100 ml beaker into the Burette through the funnel. Enter to exceed the scale of the Burette. Place a beaker containing the remainder of the fluid before it under the tap Burette. Open the tap Burette so that the liquid flows into a beaker. There must be no air bubbles on the top or bottom of the faucet tap. If all parts of the Burette was filled with liquid, then close the faucet Burette. Reinsert the existing liquid in a beaker of 100 ml under the tap Burette into the Burette through the funnel up over the scale of the Burette.

Lift the funnel and blotting paper, being careful not to blotting paper into the Burette. Note the liquid over the Burette scale and open the faucet slowly Burette so that the liquid drops. Close the faucet Burette if the liquid has reached a scale of 0.00 ml Burette. At the time of titration, at the base of a pillar or under the tap Burette, must be laid white tile or white paper so that the color change signaled the end of the titration process becomes clear.

Pipette have a level of accuracy under the Burette. Pipette used to measure fluid that Volumetric pipette and Graduated pipette. Volumetric pipette is small pipe and has a middle section oval tube as goiter. Volumetric pipette size assortment among which 1 ml, 2 ml, 5 ml, 10 ml, 20 ml, 25 ml, 50 ml and so on. Fluids are measured with the volumetric pipette is very accurate for example, if it took 5 ml of fluid with volumetric pipette 5 ml, the liquid coming out of the volumetric pipette exactly 5 ml. On top of the pipette Volumetric there is dark or blue line indicating the boundary volume pipette. Volumetric pipette tip taper so that the liquid that comes out easily in one direction.

Graduated pipette shaped like a straight pipe. Graduated pipette assortment of sizes among which 5 ml, 10 ml, 15 ml and others. The accuracy of a graduated pipette is lower when compared with the volumetric pipette. In the pipette there is a scale to 0.05 ml, for example, in graduate pipette 10 ml, there is a scale of 1 ml to 10 ml. Among a scale of 1 ml and 2 ml contained a scale of 0.1 ml to 0.9 ml. With a graduated pipette, the liquid can be measured up to 2 decimal places, for example 4.25 ml. This graduated pipette as Burette but do not have a tap at the bottom. Graduated pipette tip taper as volumetric pipette so that the liquid out easily in one direction.

How to use a pipette to take a liquid both for volumetric pipette and graduated pipette is the pipettes must be clean and have been washed and dried. If the pipette is not dry, then rinse the pipette with distilled water 3 times. And rinse with a liquid to be taken 3 times as well. This is done so that the liquid does not mix with distilled water and the concentration is reduced. After the Pipetteerballon plug at the top of the pipettes. Pipetteerballon made of rubber that is ball with three pipes. The first pipe at the top pipetteerballon to remove the air from the ball. In the pipeline there is a triangular arrow. The second pipe at the bottom like the letter t tiny of the pipetteerballon is used to draw air and suck fluids, other pipe serves to reduce the liquid. In each of the pipes there are arrows.

Attach a pipette on the bottom of pipetteerballon, enter into a pipe. And the exhaust air from the pipetteerballon by means of a ball is pressed together with the arrows on the pipe at the top of the pipetteerballon. So that the pipetteerballon will shrivel. Wipe the outside of the pipette from top to bottom using a tissue or blotting paper to dry, try do in one wipe. Open the lid of the bottle of liquid to be taken, then insert the pipette into it. Suction fluid by a squeeze of arrows on the pipe with a rubber ball that is inserted pipette.

The liquid will rise into the pipette and pipetteerballon balls will be filled with air. Take up liquid over the scale of the pipette. Then lift the pipette from the liquid and wipe the outside of the pipette from top to bottom using a tissue. Try doing in one wipe, so that pipette becomes dry. And there is no liquid from the outside pipette down and increase the volume of fluid. Place the pipette on the bottle neck where fluid before and tilt fluid bottle 45 ○ with left hand, if the right hand holding a pipette. Or vice versa.

Enforce pipettes, pipette along with Pipetteerballon hold with one hand, the index finger and thumb holding a sign triangular arrow on the pipetteerballon to reduce the liquid. Press the arrow so that the liquid down into the bottle neck. Eyes fixed on the movement of fluids, do not pass on a scale of 0.00 ml pipette. There are two curved lines inside the pipette, stop the decline of this fluid if the bottom has a curved line on a scale of 0.00 ml.

Take the pipette and still hold together with a pipetteerballon, while the hand holding the bottle neck enforce bottles and caps with a lid. For example the liquid to be taken is 5.55 ml. Take an empty 100 ml beaker with a hand that was holding the bottle and enforce the pipette. Tilt the beaker 45 ○ like the neck of the bottle. Lower fluid to 5.55 ml. The rest of the liquid in the pipette is inserted back into the bottle. Pipette which has been used should be washed again with water and then with soap and finally with water until clean. The most important part is cleaned inside the pipette. Do not dry in the oven for pipette, pipettewill expands, thereby reducing the accuracy of the pipette.